These results suggest that PS-N-BSANP enhanced the osteogenic differentiation potential in bone tissue engineering applications due to the controlled release of naringin. Furthermore, conditioned medium from PS-N-BSANP showed an increased calcium deposition and collagen matrix formation under osteogenic conditions with C3H10T1/2 cell line. In addition, sustained naringin release profile was observed from PS-N-BSANP for 12 days which showed a cumulative release of 52.54 micromolar (µM). X- ray diffraction showed amorphization of naringin in PS-N-BSANP. Further, the prepared nanoparticles and interconnected porous scaffolds were characterized by scanning electron microscopy, X-ray diffraction and fourier transform infrared spectroscopy analysis. In the present study, porous polycaprolactone scaffold entrapped with naringin loaded bovine serum albumin nanoparticles (PS-N-BSANP) has been engineered. and Research Department of Advanced Zoology and Biotechnology, Loyola College, Chennai, Tamil Nadu, India.ĪBSTRACT: A biodegradable three-dimensional scaffolds have gathered attention and are widely studied for bone tissue engineering applications. 148: 138–142.Porous Polycaprolactone Scaffold Engineered with Naringin Loaded Bovine Serum Albumin Nanoparticles for Bone Tissue Engineering Hong (2019) Improving L-serine production in Escherichia coli via synthetic protein scaffold of SerB, SerC, and EamA. Hong (2013) Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli. Hong (2018) Efficient malic acid production in Escherichia coli using a synthetic scaffold protein complex. Davies (1988) Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium. DeLisa (2008) Engineering the spatial organization of metabolic enzymes: mimicking nature’s synergy. Maranas (2001) Probing the performance limits of the Escherichia coli metabolic network subject to gene additions or deletions. Liu (2013) A serine hydroxy-methyltransferase from marine bacterium Shewanella algae: isolation, purification, characterization and l-serine production. Keasling (2009) Synthetic protein scaffolds provide modular control over metabolic flux. Nielsen (2016) Engineering of high yield production of L-serine in Escherichia coli. Tsuchiya (1990) Target of serine inhibition in Escherichia coli. Qi (2014) Construction of an L-serine producing Escherichia coli via metabolic engineering. Xu (2015) L-Serine overproduction with minimization of by-product synthesis by engineered Corynebacterium glutamicum. Eggeling (2005) Metabolic engineering of Corynebacterium glutamicum for L-serine production. Lu (2012) Construction of Escherichia coli strains producing L-serine from glucose. Nakae (2010) A 90-day feeding toxicity study of l-serine in male and female fischer 344 rats. Hirabayashi (2000) L-serine and glycine serve as major astroglia-derived trophic factors for cerebellar Purkinje neurons. These results suggest that the carbon flux was successfully directed to the L-serine secretion pathway without knocking out a competing pathway or adding expensive glycine.įuruya, S., T. By fermentation, 9.4 g/L of serine was produced at a yield of 0.34 mol/mol glucose. By the introduction of a synthetic protein scaffold without metabolic pathway engineering or addition of glycine, 1.8 g/L of L-serine was produced at pH7 and 37☌. Through this strategy, the L-serine production is more efficient than in competing pathways. A synthetic protein scaffold between SerB and EamA was introduced in this study to physically combine the two enzymes. A novel strategy is needed to overcome the L-serine degradation pathway and low L-serine tolerance of Escherichia coli for efficient L-serine production. L-serine microbial development is considered a difficult activity due to L-serine’s central role in cellular metabolism with 2 main degradation pathways. L-serine is a non-essential amino acid which has a wide range of applications and plays an important role as a building block for growing cells.
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